Table 3.
Germination results of hypnozygotes collected from co-culture of Group I only, Group III only and Group I/III isolates.
| Germination trial conditions |
||||||
|---|---|---|---|---|---|---|
| Group(s) | Co-culture | Total trials |
Overall excystment success |
Temperature (°C) |
Age at isolation (Mos.) |
Excystment success |
| I | 38-3, GTM253-17 | 1 | 2.7% | 15 | 3 | 3/110 |
|
| ||||||
| GTCN16, GTMR01 | 14 | 10.0% (22/219) | 4 | 7/15 | ||
| 6 | 1/33 | |||||
| 4 | 7 | 2/16, 1/15, 3/11, 0/8 |
||||
| 8 | 0/18* | |||||
| 9 | 3/20 | |||||
| 17 | 4/19 | |||||
|
| ||||||
| 15 | 4 | 0/21* | ||||
| 7 | 0/1, 0/21* | |||||
| 8 | 1/4 | |||||
|
| ||||||
| GTLI21, GTM253-17 | 1 | 0.0% | 15 | 3 | 0/116* | |
|
| ||||||
| 38-3, GTCN16, GTLI21, GTM253-17 | 1 | 3.0% | 15 | 3 | 5/162* | |
|
| ||||||
| III | ATSP1-B, ATSW01-1 | 1 | 2.0% | 15 | 3 | 2/100 |
|
| ||||||
| ATSW01-1, SP3B8-3 | 1 | 76.2% | 15 | 1 | 16/21 | |
|
| ||||||
| ATSW01-1, SP4B6-2 | 1 | 46.3% | 15 | 3 | 76/162 | |
|
| ||||||
| ATSW01-1, SP4E6-2 | 3 | 14.3% (4/28) | 4 | 9 | 1/5 | |
| 10 | 3/16 | |||||
|
| ||||||
| 15 | 7 | 0/7* | ||||
|
| ||||||
| SP3B8-3, SP4E3-1 | 1 | 3.8% | 15 | 4 | 1/26 | |
|
| ||||||
| SP4E5-4, SP4E6-2 | 3 | 37.5% (3/8) | 4 | 9 | 2/4 | |
|
| ||||||
| 15 | 4 | 1/3 | ||||
| 7 | 0/1* | |||||
|
| ||||||
| ATSP1-B, ATSP1-D, ATSW01-1, SP4B5-2, SP4B6-2 | 1 | 42.2% | 15 | 3 | 100/237 | |
|
| ||||||
| I, III | 2TOW1, PGT183 | 1 | 0.0% | 20 | 5 | 0/7* |
|
| ||||||
| 38-3, SP4B6-2 | 1 | 61.4% | 15 | 3 | 51/83* | |
|
| ||||||
| 38-3, GT5-6 | 1 | 0.0% | 20 | 5 | 0/19* | |
|
| ||||||
| 3TOW1, PGT183 | 1 | 0.0% | 20 | 5 | 0/9* | |
|
| ||||||
| GTCN16, SP4C7-3 | 1 | 100.0% | 4 | 6 | 1/1* | |
|
| ||||||
| GTCN16. SP5B2-3 | 6 | 10.9% (11/101) | 4 | 5 | 1/11* | |
| 6 | 0/13*, 2/18*, 5/23* |
|||||
| 9 | 2/11* | |||||
|
| ||||||
| 15 | 4 | 1/25* | ||||
|
| ||||||
| GTM253-17, ATSW01-1 | 1 | 5.1% | 15 | 3 | 5/98* | |
|
| ||||||
| GTM253-17, SP4B5-2 | 1 | 0.0% | 15 | 3 | 0/76* | |
|
| ||||||
| GTMR01, GT5-6 | 23 | 60.3% (117/194) | 4 | 6 | 1/8*, 0/10* | |
| 7 | 5/10*, 16/17*, 1/2* |
|||||
| 8 | 3/3* | |||||
| 10 | 8/9* | |||||
| 12 | 6/18*, 1/5* | |||||
| 14 | 2/2* | |||||
| 16 | 0/1* | |||||
| 18 | 9/11* | |||||
| 20 | 1/2* | |||||
| 22 | 2/5* | |||||
| 23 | 1/4* | |||||
| 30 | 1/1* | |||||
| 31 | 3/3* | |||||
| UR | 7/13* | |||||
|
| ||||||
| 15 | 6 | 0/1* | ||||
|
| ||||||
| 20 | 8 | 19/32* | ||||
| 9 | 14/19* | |||||
| 10 | 14/15* | |||||
| 15 | 3/3* | |||||
|
| ||||||
| GTMR01, SP4E5-4 | 7 | 23.1% (12/52) | 4 | 3 | 0/5* | |
| 5 | 2/4* | |||||
| 6 | 1/19*, 1/2* | |||||
| 9 | 7/14* | |||||
|
| ||||||
| 15 | 5 | 0/2* | ||||
| 7 | 1/6* | |||||
|
| ||||||
| GTMRO1, SP5B3-1 | 3 | 0.0% (0/14) | 4 | 5 | 0/3* | |
| 6 | 0/5* | |||||
|
| ||||||
| 15 | 4 | 0/6* | ||||
|
| ||||||
| MR16G6, GT5-6 | 1 | 6.7% | 20 | 5 | 1/15* | |
|
| ||||||
| 38-3, GTCN16, GTLI21, GTM253-17, ATSP1-B, ATSP1-D, ATSW01-1, SP4B5-2, SP4B6-2 |
1 | 58.4% | 15 | 3 | 115/197 | |
(*)
Excystment success is the proportion of hypnozygotes germinated (as demonstrated by the presence of germling cells or an archeopyle/empty hypnozygote test). Co-cultures that did not yield any viable hypnozygotes are shaded. Trials in which germlings did not complete more than 3 divisions are marked by an asterisk.