Figure 1.
Characterization of muscle-derived pericytes (MP) from swine. MP were analyzed for their behavior and their expression of characteristic pericyte markers to define their cellular identity. (A,B) Young (A) and old (B) swine-derived MP showing fibroblast colony-forming units (CFU-F) when seeded at low confluence. Giemsa staining on plastic 9 cm Petri dishes. (C,D) Single CFU-F morphology of young (C) and adult (D) MP, presenting identical spindle-like morphology. (E–G) Representative immunofluorescence staining of young MP showing positivity (pos) for alpha-smooth muscle actin (α-SMA) (E), chondroitin sulfate proteoglycan (NG-2) (G), and platelet-derived growth factor receptor beta (PDGFR-β) (F). Nuclei were counterstained with 4, 6-diamidino-2-phenylindole (DAPI). (H) Average data scored from immunofluorescence in (E–G). Scale bar: (A,B) 1 cm; (C–G) 15 μm.