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. 2014 May 30;5:203. doi: 10.3389/fphys.2014.00203

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Copyright © 2014 Fuoco, Sangalli, Vono, Testa, Sacchetti, Latronico, Bernardini, Madeddu, Cesareni, Seliktar, Rizzi, Bearzi, Cannata, Spinetti and Gargioli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Myogenic capability of MP in vitro. Myogenic ability was assessed for muscle-derived pericytes (MP). (A–D) Immunofluorescence staining with anti-myosin heavy chain (MyHC) antibody. Representative images showing MyHC-positive (pos) myotubes (red) formed from young (A,B) and old (C,D) MP, demonstrating that age affects MP muscle differentiation capacity. Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI). (E) Average data extracted from (A–D) immunolabeling. The number of positive fibers was quantified with respect to the total number of nuclei. The efficiency of cell fusion into syncytial myofibers was measured as percentage of nuclei per fiber. The analysis was done on five pictures from three donor/group. Values are expressed as mean ±s.e.m. ^*p < 0.05 vs. young MP. Scale bar: (A–D) 50 μm.