Fig. 2.

Analysis of DNA content of cells treated with Taxol, discodermolide, or both for 48 hours. *, the concurrent combination potentiated drug-induced aneuploidy at low doses of either drug (≤ 20 nmol/L). These concentrations resulted in synergistic cytotoxicity, as determined in Fig. 1. Higher doses of Taxol and discodermolide in combination resulted in minimal alterations to the cell cycle profile, relative to single agent treatment. These higher doses (≥100 nmol/L) induced mitotic arrest both alone and in combination and equate to additive/antagonistic cytotoxicity.