Skip to main content
. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Structure. 2014 Mar 27;22(5):697–706. doi: 10.1016/j.str.2014.03.002

Figure 3. Actin binding to Vcn is necessary for the mechanical response to force on integrins.

Figure 3

Upon applying pulses of constant force, a decrease in the relative bead displacement of Vin−/− MEFs transfected with VcnWT is observed, in contrast to the increase observed for Vin−/− MEFs transfected with VcnI997A. Two force pulses were applied to FN-coated beads bound to Vin−/− MEFs transfected with either VcnWT (n=20), VcnV1001A (n=19) or VcnI997A (n = 26) and displacement was measured. * indicates a p-value< 0.05. *** indicates a p-value<0.001. Error bars are ± SEM. These results indicate that actin binding to Vcn plays a role in Vcn’s ability to respond to force.