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. 2014 Mar 31;5(7):1897–1911. doi: 10.18632/oncotarget.1876

Figure 5. γ-tocotrienol induces apoptosis and inhibits AKT/mTOR pathway in HCC cells.

Figure 5

A, γ-tocotrienol inhibited cell viability of HCCLM3 cells in a dose dependent manner. Cell viability was quantified by MTT assay. Columns, mean from three different experiments; bars, SD. B, γ-tocotrienol induces apoptosis in HCCLM3 cells in a time-dependent as evident by western blot analysis of PARP. HCCLM3 cells were treated with 50 μM γ-tocotrienol for the indicated times, and whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to western blot analysis against PARP antibody. The same blot was stripped and reprobed with β-actin antibody to show equal protein loading. C, γ-tocotrienol suppressed the phosphorylation of mTOR signaling pathway kinases in HCCLM3 cell. HCCLM3 cells were treated with 50 μM γ-tocotrienol for the indicated times, and whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to western blot analysis against phosphor-specific antibodies for AKT, mTOR and P70S6K1. The same blots were stripped and reprobed with antibodies for AKT, mTOR and P70S6K1 to show equal protein loading. The results shown are representative of two independent experiments.