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Coupled In Vitro Analysis of Aminotransferase Activities of Individual GenB Enzymes
(A) Amination of 6′-DOG generated in situ from G418 by the reaction of GenQ.
(B) Amination of 6′-DOX generated in situ from gentamicin X2 by the reaction of GenQ; Reaction mixtures were analyzed after incubation for 16 hr. The amino donor was L-methionine.
See also Figures S6 and S7.
