Figure 3. In vitro T-cell Production of IFN-γ.

(A) and (B) BM-resident T cells were classified as effector memory (T_EM), central memory (C_M), naïve (N) or effector (T_EFF) T cells based on the expression pattern of CD45RA and CD62L, as previously reported [36]. Antigen expression patterns in two representative BM samples from children with IDO-expressing AML are shown. BM MNC were first gated on CD4⁺ or CD8 T cells, followed by the quantification of CD62L/CD45RA expression. Quadrant markers were set according to the proper isotypic control; (C) and (D) Production of IFN-γ at the single-cell level in 2 representative BM samples from IDO-positive AMLs. Details on culture conditions were provided in materials and Methods. BM MNC were first gated on CD4⁺ or CD8⁺ T cells, followed by the quantification of IFN-γ, IL-4 and IL-17 expression. Markers were set according to the proper isotypic control. The percentage of cells staining positively for a given antigen is indicated.