Figure 3. Ectopic Ring1B is able to induce Fak expression and cell migration.

A. Ectopically expressed Ring1B enhances Fak expression, as determined by western blot. Lamin B, loading control. Numbers at the bottom of each western blot lane represent protein band intensities normalized to Lamin B and relative to control cells, as described in “Methods”. B. Focal contact formation in Ring1B overexpressing NMuMG cells, determined by immunofluorescence to detect Fak. Bars, 10 μm. C. Ectopic Ring1B expression results in NMuMG cell migration, determined by scratch assays. Graph shows data (mean ± SD) from a wound healing assay of NMuMG cells transfected with empty or Ring1B expression vectors. Bars, 2 mM. Experiment was performed in triplicate and repeated three times with similar results. **, P < 0.005.