Skip to main content
. 2014 Mar 23;5(8):2131–2148. doi: 10.18632/oncotarget.1852

Fig 2. Inhibition of CK2 blocks IL-6 family induced STAT signaling.

Fig 2

Serum-starved HepG2 or HeLa cells were stimulated with the indicated cytokines for 15 min: (A) HepG2: 10 ng/ml IL-6; HeLa: 20 ng/ml IL-6, (B) HepG2: 10 ng/ml IL-11; HeLa: 20 ng/ml IL-11, (C) 10 ng/ml Hyper-IL-6, (D) 10 ng/ml IL-27, (E) 10 ng/ml LIF, (F) 10 ng/ml CT-1 and (G) 10 ng/ml OSM. The cells were pre-incubated with the CK2 inhibitor TBB (100 μM) for 90 min where indicated. Phosphorylation of STAT1 and STAT3 was assessed by Western blotting, and STAT1/STAT3 served as internal loading control, respectively. One representative Western blot from at least three independent experiments is shown.