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. 2014 Apr 16;5(8):2276–2292. doi: 10.18632/oncotarget.1902

Figure 4. ANRIL could bind to PRC2, and subcellular fractionation location of ANRIL.

Figure 4

(A) RIP experiments were performed in SGC-7901 and BGC-823 cells and the coprecipitated RNA was subjected to qRT-PCR for ANRIL. HOTAIR was used as a positive control. The fold enrichment of ANRIL in EZH2 RIP is relative to its matching IgG control RIP. (B) The fold enrichment of ANRIL in SUZ12 RIP is relative to its matching IgG control RIP in SGC-7901 and BGC-823 cells. (C) ANRIL nuclear localization, as identified using qRT-PCR in fractionated SGC-7901 and BGC-823 cells. After nuclear and cytosolic separation, RNA expression levels in SGC-7901 and BGC-823 cells were measured by qRT-PCR. GAPDH was used as a cytosol marker and U6 was used as a nucleus marker. *, P < 0.05, **, P < 0.01.