Representative ER IHC staining in two separate carcinomas (10x). Sections A and C are each from carcinoma #1; B and D are each from carcinoma #2. All stains were performed at Mayo.
ER IHC staining using single ER antibody (1D5) is depicted in panels A and B. ER IHC staining using the dual ER antibody cocktail (1D5/2, 123) is depicted in panels C and D. Each carcinoma shows negative staining with single ER antibody (A, B) and positive staining by dual antibody cocktail(C, D).