Skip to main content
. 2013 Nov 15;5(6):1458–1474. doi: 10.18632/oncotarget.1538

Figure 2. CDH11 regulates colony growth and proliferation of MDA-MB-231 breast cancer cells and PC-3 prostate cancer cells.

Figure 2

(A) Western blot analysis of CDH11 in MDA-MB-231 cells stably expressing CDH11 shRNA (33 or 34 clonal cells) or siRNA (4A or 6A pooled cell lines). (B) Effect of CDH11 depletion on proliferation of MDA-MB-231 cells measured using crystal violet staining after 5 days. (C) Effect of CDH11 depletion on anchorage-independent colony formation in soft agar. (D) Western blot analysis of CDH11 in PC3 cells CDH11 shRNA, (E) CDH11 knockdown fails to significantly reduce the proliferation of PC3 cells but (F) colony formation is significantly reduced upon CDH11 depletion. (EV, 1B): Empty vector. (Luc) scrambled control. GAPDH was used as a loading control for western blot. Phase image using a 4x objective on a Zeiss inverted microscope. Data are presented as means ± SEM (Student's t-test).