Figure 6. 4-[5-(2,5-Dimethylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzene sulfonamide (DMC) significantly reduced the proliferation of MDA-231 cells but had no effect on CDH11-negative MCF7 cells in vivo.

Nude mice were implanted with 6x10⁶ MDA231 (N=4/cohort) or MCF7 cells (N=3/cohort) in the mammary fat pad. Once the tumors became palpable, animals were treated with (150 mg/kg/day) of DMC or vehicle for 48 hours and euthanized 2 hours post final treatment. (A) Representative ki67 immunostaining (proliferation marker), TUNEL (apoptosis marker) and corresponding H&E sections from xenografts. (B) DMC significantly lowered the proliferation rate of MDA231 transplanted cells but not MCF7 cells. (C) DMC did not have any significant effect on the apoptosis rate of cancer cells. Arrows indicate representative proliferative cells. Black arrow-heads demonstrate apoptotic cells and red arrow-heads indicate mitotic-figures. Scale bar= 20 micrometers. Columns and bars show the mean and ESM respectively (student's t-test). ***p=0.0006