Figure 3. c-kit cooperated with activated oncogenic Ras to promote tumorigenesis.

ACC-M cells were infected with empty vector, retroviral vector encoding -kit, or sequentially infected with retroviral vector encoding H-RasV12G (Ras) and c-kit as indicated. A), Phase contrast images of the cell morphology. Scale bar, 100 mm. B), Immunoblotting analysis of expression of the epithelial and mesenchymal markers. C and D,E,F), Migration (C and D) and invasion (E and F) assays. The mean was derived from cell counts of 5 fields, and each experiment was repeated 3 times. Representative images of migrated or invaded cells are also shown. G), Individual tumor volume was measured according to the formula: Л/6 × length ×width² at the 7^th week after injection.