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. 2014 Mar 12;5(6):1657–1665. doi: 10.18632/oncotarget.1823

Figure 1. Differential growth of heterogeneous glioma cell lines.

Figure 1

A. Parental cultures were subcloned in soft agar to establish non-adherent spheroid (NS) and serum-adherent (SA) subpopulations that were subsequently maintained under defined yet distinct culture conditions. B. Phase contrast images showing the morphologic differences of cells grown under various culture conditions. C. Comparison of colony formation in soft agar containing bovine serum (BS) or growth factors (GFs) for parental (P) and NS subcultures of U251 and A172. Error bars correspond to SEM derived from 4 replicates. D. Differentiation of NS subcultures derived from U251 and A172 cell lines showing changes in nestin (NES, green) and GFAP (red) expression by immunohistochemistry. The ratio of fluorescent intensity (NES/GFAP) quantified by ImageJ is shown to the right of each paired panel.