Table 1.
NWD1 interacting proteins identified by proteomic analysis. LNCaP cells in T-75 flasks (70% confluency), cultured in standard RPMI 10% FBS supplemented with 0.5 nM R1881 (to stimulate cell growth), were transfected by TransIT-2020 reagent (Mirus) with 10 μg of FLAG-GFP (control) or FLAG-NWD1 expression constructs. At 96 hours post-transfection, cells were lysed for further immunoprecipitation (IP) procedures using anti-FLAG beads. Mass spectrometry (1D LC-MS/MS) was performed twice using respective IP eluates. Protein hits also present in the control sample were subtracted to point out specific NWD1-related hits. Each protein entry shows the respective IPI (International Protein Index) number and the total protein spectral counts. The respective protein functions are also listed.
| IPI# | Gene Symbol | Protein Identified | Spectral Count | Function |
| IPI00744647 | NWD1 | NACHT and WD repeat domain-containing protein 1 | 401 | |
| IPI00007752 | TUBB2C | Tubulin beta-2C chain | 99 | microtubule structure |
| IPI00941328 | HSPA1B | Heat shock 70kDa protein 1B | 92 | chaperone |
| IPI00382470 | HSP90AA1 | Heat shock protein HSP 90-alpha | 54 | chaperone |
| IPI00414676 | HSP90AB1 | Heat shock protein HSP 90-beta | 50 | chaperone |
| IPI00292496 | TUBB8 | Tubulin beta-8 chain | 42 | microtubule structure |
| IPI00015947 | DNAJB1 | DnaJ (Hsp40) homolog subfamily B member 1 | 22 | co-chaperone |
| IPI00008868 | MAP1B | Microtubule-associated protein 1B | 21 | microtubule assembly |
| IPI00000643 | BAG2 | BAG family molecular chaperone regulator 2 | 11 | co-chaperone |
| IPI00480014 | SSX11 | Synovial sarcoma X (SSX) breakpoint protein 11 | 10 | transcriptional modulation |
| IPI00030275 | TRAP1 | Heat shock protein 75 kDa, mitochondrial | 8 | chaperone |
| IPI00000690 | AIFM1 | Apoptosis-inducing factor 1, mitochondrial | 7 | regulation of apoptosis |
| IPI00020058 | ATP7B | Copper-transporting ATPase 2 | 4 | copper transport |
| IPI00554648 | KRT8 | Keratin, type II cytoskeletal 8 | 4 | cytoskeleton structure |
| IPI00015842 | RCN1 | Reticulocalbin-1 | 3 | calcium binding |
| IPI00021187 | RUVBL1 | RuvB-like 1 | 3 | transcriptional modulation |
| IPI00029628 | RCN2 | Reticulocalbin-2 | 3 | calcium binding |
| IPI00064328 | PRMT5 | Protein arginine N-methyltransferase 5 | 3 | metabolic modulation |
| IPI00440493 | ATP5A1 | ATP synthase subunit alpha, mitochondrial | 3 | ATP synthesis |
| IPI00032406 | DNAJA2 | DnaJ (Hsp40) homolog subfamily A member 2 | 2 | co-chaperone |
| IPI00909055 | KLHDC3 | Kelch domain-containing protein 3 | 2 | meiotic recombination |