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editorial
. 2014 Apr 2;5(6):1683–1698. doi: 10.18632/oncotarget.1849

Figure 3. Strigolactone analogues induce cell death via apoptosis.

Figure 3

A, HCT116 cells were treated with the indicated concentrations of SLs for 24 hr. Cells were co-stained with annexin-V and PI and analyzed by flow cytometry. The distribution of HCT116 cells in early (Annexin-/PI+, gray bars) and late (Annexin+/PI+, black bars) apoptosis following SL treatment. B. Cell cycle distribution of DU145 cells treated with the indicated doses of MEB55 for 48 hr, washed with PBS and overlaid with fresh media without SL for additional 24 hrs and analyzed by flow cytometry. C-E. Assay of SL reversibility in cells. HCT116 (C), DU145 (D) and U2OS (E) cells were treated with the indicated SLs for the indicated times, the cells were washed with PBS and overlaid with fresh DMEM containing 10% FBS minus SLs. Changes in cell viability were assessed by an XTT assay and are shown as mean ±SD of triplicate wells and expressed as percent of control.