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Northern analysis confirms predominantly independent expression of Mtb regX3 during Pi depletion. Biotinylated single-strand regX3 probe (247 bp) was used for hybridization. Lane 1, BrightStar biotinylated RNA millennium marker (Ambion). Lane 2, total Mtb RNA following 72 h of Pi depletion. White arrow indicates a band corresponding to the independently expressed regX3 transcript (~0.8 kb). The experiment was repeated using two biological samples with the same results.
