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. 2014 Jun;160(Pt 6):1125–1133. doi: 10.1099/mic.0.077180-0

Fig. 5.

Fig. 5.

Preserved expression of regX3 by RT-PCR in a senX3-disrupted Mtb mutant strain during Pi depletion. Total RNA from 24 h Pi-depleted cultures of a mutant strain containing a Tn insertion in senX3 (senX3 : : Tn) and the isogenic wild-type was used for cDNA synthesis with oligo(dT)20 primer followed by PCR amplification. (a) Illustration of primer pairs used to amplify cDNA corresponding to the senX3 transcript (senX3-F/senX3-R) and regX3 transcript (regX3-F/regX3-R), and the senX3-regX3 co-transcript (SR-F/SR-R). The Tn insertion is at bp 162 in the senX3 gene of senX3 : : Tn (grey triangle). (b) RT-PCR results. Lanes: 1100 bp DNA marker (Fermentas); 2, senX3 expression in wild-type; 3, co-transcript expression in wild-type; 4, regX3 expression in wild-type; 5, senX3 expression in senX3 : : Tn; 6, co-transcript expression in senX3 : : Tn; 7, regX3 expression in senX3 : : Tn.