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. 2014 May 8;53(20):3267–3277. doi: 10.1021/bi500427r

Figure 1.

Figure 1

SEM1(86–107) sequence and pH dependence of fibril formation. (A) SEM1(86–107) peptide sequence. Acidic residues are colored red, basic residues blue, and His residues purple. (B) SEM1(86–107) fibril formation was assessed by ThT fluorescence following incubation for 24 h in PBS pH 7.7 (black line) or PBS 2.5 (red line). (C) Far-UV CD spectra were measured prior to (blue line) and following (black and red lines) SEM1(86–107) fibril formation at pH 7.7 (blue and black lines) and 2.5 (red line). (D and E) SEM1(86–107) fibril formation was also assessed by AFM imaging after incubation for 24 h in PBS pH 7.7 (D) or PBS 2.5 (E). Scale bars in panels D and E correspond to 500 nm.