Table 2.
Summary of differential protein spots of urine between patients with UTUC and healthy people identified by LC-MS/MS
| Spot no | Protein name | Accession no | Calculate Mw/ pI | Peptide matched | Sequence covered% | MASCOT score |
|---|---|---|---|---|---|---|
| 1 |
ATP-binding cassette sub-family A member 9 |
Q8IUA7 |
184.2/6.49 |
10 |
1 |
38 |
| 2 |
sorting nexin-14 |
Q9Y5W7 |
102.8/6.4 |
17 |
3 |
34 |
| 3 |
serum albumin precursor |
P02768 |
69.3/5.92 |
1 |
2 |
57 |
| 4 |
F-box/WD repeat protein 1A |
Q9Y297 |
68.8/8.3 |
29 |
3 |
33 |
| 5 |
spermatogenesis-associated protein 7 |
Q9P0W8 |
67.6/5.9 |
3 |
4 |
34 |
| 6 |
actin |
P62736 |
41.9/5.23 |
2 |
9 |
45 |
| 7 |
cytokeratin 1 |
P04264 |
65.9/8.16 |
3 |
3 |
40 |
| 8 |
DNA replication licensing factor MCM2 |
P49736 |
101.8/5.34 |
40 |
1 |
50 |
| 9 |
proline-serine-threonine phosphatase-interacting protein 1 |
O43586 |
47.5/5.53 |
6 |
4 |
40 |
| 10 |
X box-binding protein 1 |
P17861 |
28.6/9.71 |
18 |
3 |
35 |
| 11 |
immunoglobulin kappa chain C region |
P01834 |
11.6/5.58 |
16 |
49 |
151 |
| 12 |
immunoglobulin kappa chain V-I region AG |
P01593 |
11.9/5.67 |
4 |
31 |
176 |
| 13 |
hemopexin precursor |
P02790 |
51.6/6.55 |
4 |
7 |
86 |
| 14 |
prostaglandin-H2 D-isomerase precursor |
P41222 |
21.0/7.66 |
3 |
12 |
33 |
| 15 |
immunoglobulin kappa chain C region |
P01834 |
11.6/5.58 |
22 |
49 |
217 |
| 16 |
gelsolin precursor |
P06396 |
85.6/5.9 |
9 |
7 |
208 |
| 17 |
SH3-containing GRB2-like protein 3 |
Q99963 |
39.2/5.27 |
16 |
8 |
32 |
| 18 |
hemoglobin subunit beta |
P68871 |
15.9/6.75 |
9 |
32 |
214 |
| 19 |
inter-alpha-trypsin inhibitor heavy chain H4 |
Q14624 |
103.2/6.51 |
13 |
10 |
243 |
| 20 |
cytokeratin 10 |
P13645 |
59.4/5.13 |
2 |
4 |
38 |
| 21 |
basement membrane-specific heparan sulfate proteoglycan core protein precursor |
P98120 |
468.8/6.06 |
116 |
4 |
2114 |
| 22 |
retinol Binding Protein 4 |
P02753 |
22.9/5.76 |
45 |
35 |
233 |
| 23 |
Zn-alpha2-glycoprotein |
P25311 |
34.7/5.71 |
14 |
45 |
378 |
| 24 |
Zn-alpha2-glycoprotein |
P25311 |
34.7/5.71 |
5 |
16 |
98 |
| 25 |
fibrinogen gamma chain precursor |
P02679 |
51.4/5.37 |
19 |
24 |
201 |
| 26 |
fibrinogen gamma chain precursor |
P02679 |
51.4/5.37 |
5 |
11 |
38 |
| 27 |
serotransferrin precursor |
P02787 |
77.0/6.81 |
7 |
5 |
48 |
| 28 |
serotransferrin precursor |
P02787 |
77.0/6.81 |
18 |
13 |
88 |
| 29 |
annexin A3 |
P12429 |
36.3/5.63 |
3 |
4 |
37 |
| 30 |
dermcidin precursor |
P81605 |
11.2/6.08 |
2 |
16 |
48 |
| 31 |
calreticulin |
P27797 |
48.1/4.29 |
19 |
36 |
493 |
| 32 |
haptoglobin |
P00738 |
45.1/6.13 |
39 |
13 |
432 |
| 33 |
haptoglobin |
P00738 |
45.1/6.13 |
41 |
20 |
466 |
| 34 |
haptoglobin |
P00738 |
45.1/6.13 |
49 |
17 |
668 |
| 35 |
haptoglobin |
P00738 |
45.1/6.13 |
19 |
15 |
271 |
| 36 |
haptoglobin |
P00738 |
45.1/6.13 |
5 |
13 |
77 |
| 37 |
glial fibrillary acidic protein |
P14136 |
49.8/5.42 |
2 |
4 |
43 |
| 38 |
alpha-1-microglobulin/bikunin precursor |
P02760 |
38.9/5.95 |
41 |
14 |
698 |
| 39 |
alpha-1-microglobulin/bikunin precursor |
P02760 |
38.9/5.95 |
48 |
15 |
732 |
|
Spot no |
Protein name |
Accession no |
Calculate Mw/
pI
|
Peptide matched |
Sequence covered% |
MASCOT score |
| 40 |
alpha-1-microglobulin/bikunin precursor |
P02760 |
38.9/5.95 |
45 |
23 |
667 |
| 41 |
syntaxin-binding protein 4 |
Q6ZWJ1 |
61.7/5.16 |
4 |
7 |
40 |
| 42 |
mRNA cap guanine-N7 methyltransferase |
O43148 |
54.8/6.3 |
11 |
3 |
52 |
| 43 |
transthyretin precursor |
P02766 |
15.8/5.52 |
13 |
48 |
217 |
| 44 |
transthyretin precursor |
P02766 |
15.8/5.52 |
8 |
40 |
142 |
| 45 |
alpha-1-microglobulin/bikunin precursor |
P02760 |
38.9/5.95 |
5 |
11 |
63 |
| 46 |
alpha-1-microglobulin/bikunin precursor |
P02760 |
38.9/5.95 |
13 |
11 |
198 |
| 47 |
transthyretin precursor |
P02766 |
15.8/5.52 |
4 |
14 |
46 |
| 48 |
serine/threonine-protein kinase MRCK beta |
Q9Y5S2 |
194.1/5.91 |
3 |
2 |
56 |
| 49 |
serum albumin precursor |
P02768 |
69.3/5.92 |
51 |
25 |
594 |
| 50 |
serotransferrin precursor |
P02787 |
77.0/6.81 |
42 |
21 |
441 |
| 51 |
serum albumin precursor |
P02768 |
69.3/5.92 |
92 |
26 |
1359 |
| 52 |
serum albumin precursor |
P02768 |
69.3/5.92 |
48 |
26 |
662 |
| 53 |
annexin A2 |
P07355 |
38.58/7.57 |
28 |
37 |
505 |
| 54 |
Ig gamma-1 chain C region |
P01857 |
36.0/8.46 |
20 |
26 |
190 |
| 55 | Ig gamma-1 chain C region | P01857 | 36.0/8.46 | 12 | 25 | 104 |