Figure 4.

(a) Effect of incubation on the frequency of the long-lived DNA duplex events and effect of matrix components on the (b) mean residence time and (c) frequency of the aLF-cDNA duplex events. The experiments were performed at +180 mV using the (M113F)₇ αHL pore in a solution comprising 1.0 M NaCl and 10 mM Tris·HCl (pH 7.5). In the experiment with the unincubated aLF sample, the electrolyte solution contained an additional 20-base ssDNA (sequence 5′-TCAATATTTAACAATAATCC-3′) as the molecular probe. The concentrations of HSA, aLF, T₂₀, and cDNA probe used in experiments shown in panels b and c were 10 μg/mL, 100 nM, 100 nM, and 1 μM, respectively.