Figure 4.

The effect of insulin ramp stimulation on glucose metabolism. (A) Time courses of pAKT, F16P, PEPCK, glycogen and GLC_ex in response to a step stimulation (red), a ramp stimulation (blue) and 0 nM (black) of insulin in FAO cells. The mean values and SEMs of three independent experiments are shown. The step and ramp stimulations of insulin are shown at the bottom. The final concentrations of step and ramp stimulation of insulin were set at 0.1 nM, which mimics the basal secretion of insulin in vivo (Polonsky et al, 1988b; Basu et al, 1996). Note that the scale of absolute concentration of GLC_ex was normalised because of the variation between experiments. (B) Time courses of pAKT, PEPCK, glycogen, GLC_ex and F16P in response to a ramp stimulation of the insulin-dependent glucose metabolism model. Source data for this figure is available on the online supplementary information page.

Source data for Figure 4A ^{(1.3KB, txt)}