Figure 2.

Physical organization of the SUMO system. (A) Nup2 is required for proper Ulp1 localization. Ulp1^150–621GFP is mislocalized in nup60, mlp1/mlp2, esc1 and nup2 deletants, but not in nup59, nup100 or nup188 deletion strains. Scale bar=5 μm. (B) Localization of GFP-tagged SUMO system components relative to the RFP-tagged nucleolar marker Nop2. (C) Cells were co-transfected with the indicated plasmids and imaged, as above. (D) Summary of localization for each SUMO system component. DAPI, 4′,6-diamidino-2-phenylindole; scale bar=5 μm.