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. 2014 May 29;55(5):3320–3327. doi: 10.1167/iovs.13-13779

Figure 4.

Figure 4

Angiopoietin-2 expression and depletion in LECs. (A, B) Reverse transcription-PCR analysis and immuncytofluorescent microscopic analysis of Ang-2 expression in LECs. Angiopoietin-2: red. DAPI nuclear staining: blue. Scale bar: 50 μm. (C) Reverse transcription-PCR assay showing Ang-2 gene depletion in LECs 48 hours following transfection with Ang-2 siRNA, as compared with scrambled siRNA. (D) Summarized data showing significant inhibition of LEC proliferation following transfection with Ang-2 siRNA, as revealed by MTS proliferation assay. ***P < 0.001. (E) Representative micrographs showing significant suppression of LEC capillary tube formation in matrigel following transfection with Ang-2 siRNA. Scale bars: 100 μm. (F) Summarized data on total tubule length measurement. **P < 0.01.