. Author manuscript; available in PMC: 2015 Jun 1.

Published in final edited form as: J Biol Inorg Chem. 2014 Mar 11;19(0):491–504. doi: 10.1007/s00775-014-1122-9

Table 1.

Steady-state kinetic parameters of HPCDs and MndDs.

Enzyme	K_M^HPCA (μM)	K_M^O2 (μM)	k_cat/[metal] (min⁻¹)	k_cat/K_M^HPCA (μM⁻¹ min⁻¹)	k_cat/K_M^O2 (μM⁻¹ min⁻¹)
Fe-HPCD	31 ± 6	60	470 ± 20	15 ± 2	7.8 ± 0.3
Mn-HPCD	35 ± 5	50 ± 4	370 ± 10	11 ± 2	7.4 ± 0.6
Co-HPCD	5 ± 1	1200 ± 100	215 ± 8^a 590 ± 20^b 1120 ± 70^c	43 ± 9 120 ± 20 220 ± 50	0.18 ± 0.02 0.49 ± 0.05 0.9 ± 0.1
Mn-MndD	14 ± 4	62	360 ± 20	25 ± 4	5.8 ± 0.3
Fe-MndD	15 ± 4	37 ± 6	420 ± 20	28 ± 4	11.4 ± 1.4

Data from refs [8, 9, 13], measured under ambient O₂ at 22 °C in 50 mM MOPS pH 7.8; unless otherwise noted.

measured in O₂-saturated buffer at 22 °C.

V_max extrapolated from Figure 3 of ref. [9].