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. 2014 May 30;9(5):e96693. doi: 10.1371/journal.pone.0096693

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© 2014 Lund et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Confocal laser microscopy of fixed U-2-OS cells transiently expressing fluorescent proteins localized to major cellular compartments. Shown are representative images of eYFP or eGFP detected 48 hours after transfection: (A1-3) pFAST6-eYFP::NLS, (B1-3) pFAST5-eGFP::PTS1, (C1-3) pFAST37-eGFP::c-Ha-Ras, (D) pFAST57-CRT::eGFP::KDEL, (E) pFAST58-COXVIII::eGFP, (F) pFAST59-GalNAcT1::eGFP, (G) pFAST61-b1,4GT::eGFP, (H) pFAST56-a-2,6ST-eGFP. (A1–H1) microscopy with fluorescence filters. (A2–H2) nuclei stained with DAPI (dark blue). (A3–H3) merged pictures.