Table 3. Elements included in the pBASE and pFAST vector platforms.

Category	Element	Template	Reference/Source
Promoters	CMV	pcDNA3.1(+)	Invitrogen
	PGK	pSUPERIOR.puro	Oligoengine
	SV40	pcDNA3.1(+)	Invitrogen
Terminators	BGH pA	pcDNA3.1(+)	Invitrogen
	SV40 pA	pcDNA3.1(+)	Invitrogen
	hGH pA	pFLAG-CMV	Sigma
Marker cassettes	HygR	pcDNA3.1/Hygro(+)	Invitrogen
	NeoR	pcDNA3.1(+)	Invitrogen
	DHFR	pIRES-DHFR	In house/GenBank: BC005796
Targeting signals	NLS (nuclear)	Synthetic	[17]
	PTS1 (peroxisomal)	Synthetic	[18]
	c-Ha-ras (plasma membrane)	Synthetic	[19]–[20]
	COX-VIII (mitochondrial)	Synthetic	[21]
	CRT (ER)	Synthetic	[22]
	KDEL (ER retention signal)	Synthetic	[23]
	GalNacT1 (medial-Golgi)	Synthetic	[24]
	β-1,4 GT (trans-Golgi)	Synthetic	[25]
	α-2,6 ST (TGN)	Synthetic	[26]–[27]
Secretion signals	hIFN-γ	Synthetic	[28]–[29]
Proteins	eGFP	peGFP-1	Clontech
	eYFP	peYFP-C1	Clontech
	eCFP	peCFP-C1	Clontech
	mCherry	pmCherry-N1	Clontech
	SEAP	pGEM-4Z/PLAP489	[14]
Other elements	IRES	pIRES-DHFR	In house
	Vector backbone	pU0002	[13]
	His-tag	Synthetic	[30]
	FLAG-tag	Synthetic	Invitrogen
	c-Myc-tag	Synthetic	Invitrogen

α-2,6 ST: N-terminal targeting signal of beta-galactoside alpha-2,6-sialyltransferase; β-1,4 GT: N-terminal targeting signal of beta-1,4-galactosyltransferase; c-Ha-ras: C-terminal targeting signal of c-Ha-ras p21 protein; COX-VIII: N-terminal targeting signal of cytochrome c oxidase subunit VIII; CRT: N-terminal targeting signal of calreticulin; ER: endoplasmic reticulum; GalNAcT1: N-terminal targeting signal of N-acetylgalactosaminyltransferase; hIFN-γ: human Interferon-gamma; NLS: C-terminal nuclear localization sequence; PTS1: C-terminal peroxisomal target signal 1; TGN: trans-Golgi network.