Figure 4.

Immunofluorescence staining of the alveolar type-II cell marker SPC and alveolar type-I cell marker T1α in recellularized lung tissues. Decellularized lung scaffolds were reseeded with mouse fibroblasts, undifferentiated iPSCs, or differentiated iPSCs and immunostained with goat anti-mouse pro-SPC or T1α antibodies after 12 days. Yellow arrows indicate SPC⁺ or T1α⁺ cells. Bottom panels show magnified views of the areas marked by the dotted lines in the top panels. Scale bars = 50 μm (top panels), 10 μm (bottom panels). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; iPSC, induced pluripotent stem cell; SPC, surfactant protein C.