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. 2014 Apr 24;3(6):675–685. doi: 10.5966/sctm.2013-0142

Figure 7.

Figure 7.

Effect of differentiated iPSC transplantation on the BALF profile in BLM-treated mice. Following intratracheal exposure to normal saline or 4 U/kg BLM, fibroblasts, undifferentiated iPSCs, or differentiated iPSCs were instilled intratracheally into mice on day 2. Bronchoalveolar lavage was performed on day 12. (A): BALFs from the four groups were cytospun, and the cell pellets were stained using Diff-Quik. Representative images are shown. Yellow arrows indicate neutrophils, and black arrows indicate lymphocytes. Scale bars = 50 μm. IL-6 (B) and TNF-α (C) concentrations in BALFs were measured using sandwich enzyme-linked immunosorbent assay kits. Data are expressed as the means ± SD; n ≥ 5 per group. (B): ∗, p < .05 versus the saline control group; ▴, p > .05 versus the BLM group and BLM/fibroblast group; #, p < .05 versus the BLM group; †p > .05 versus the saline control group. (C): ∗∗, p < .01 versus the saline control group; ∗, p < .05 versus the saline control group; ▴, p > .05 versus the BLM group and BLM/fibroblast group; #, p < .01 versus the BLM group; p < .05 versus the BLM/fibroblast group; †, p > .05 versus the saline control group. Abbreviations: BALF, bronchoalveolar lavage fluid; BLM, bleomycin; IL-6, interleukin-6; iPSC, induced pluripotent stem cell; TNF, tumor necrosis factor.