Figure 2. Anterograde and retrograde velocities of GFP/YFP tagged vesicles/cargo significantly change temporally.

The average duration weighted segmental velocities of six vesicles/organelle revealed significant shifts in their movement dynamics at day 2 compared to day 1. Box-plots outline the distribution of vesicle velocities. Upper and lower box edges represent 75^th percentile and 25^th percentile respectively. Upper and lower whiskers represent the maximum and minimum of vesicle velocities respectively. The horizontal line represents the median of vesicle velocities. (A) The anterograde duration-weighted segmental velocity of APP-YFP vesicles significantly increased at day 2 (p = 0.015). Anterograde segmental velocity distributions of ANF-GFP vesicles also significantly increased at day 2 (p = 1.068E-6). Strikingly, anterograde segmental velocity of SYNB-GFP vesicles and SYNT-GFP vesicles significantly decreased over time (p = 3.484E-6 and p = 0.0004, respectively). HTFR-GFP and MITO showed no significant change at day 2 compared to day 1. (B) The retrograde duration-weighted segmental velocity of ANF-GFP significantly increased (p = 1.092E-7), while SYNB-GFP significantly decreased over time (p = 6.043E-7). APP-YFP, HTFR-GFP, SYNT-GFP, and MITO-GFP showed no significant changes at day 2 compared to day 1. N = 10 cells; *p<0.05, **p<0.01, ***p<0.001 by Wilcoxon-Mann-Whitney rank sum test for nonparametric distributions. NS = not significant.