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. 2014 May 30;9(5):e98149. doi: 10.1371/journal.pone.0098149

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© 2014 Rollin-Pinheiro et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) ELISA analysis of the binding of Mabs to S. apiospermum CMH. The amount of antibody bound to CMHs was determined by incubation with a rabbit anti-mouse IgG. CMH from S. apiospermum (closed circle); CMH from A. fumigatus (closed square); CMH from S. apiospermum + unrelated antibody (open circle); negative control (open square). Inset: HPTLC of S. apiospermum CMH (spots a,b,c), which was developed in CHCl3: MeOH: 2 M NH4OH (40:10:1 v/v). Lane 1: stained with orcinol/ H2SO4; Lane 2: immunostaining with the anti-CMH MAb. Indirect immunofluorescence analysis of conidial (B and C) and mycelial (D and E) forms of S. apiospermum using the anti-CMH MAb. B and D: phase contrast; C and E: fluorescence signal.