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. 2014 May 30;9(5):e98149. doi: 10.1371/journal.pone.0098149

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© 2014 Rollin-Pinheiro et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cytotoxic assay of S. apiospermum CMH on L929 and RAW cells during 48 h of incubation. Cell viability was measured by adding a neutral-red solution and measuring the absorbance at 490 nm using a spectrophotometer. The presence of S. apiospermum CMH in the culture supernatant was analyzed using TLC.