Table 2. Detection of Phialophora gregata in stems with PCR, observation of hyphae in vessels, and stem symptom severity of resistant and susceptible cultivars at 2 and 6 weeks post inoculation.

		2 Weeks Post Inoculation				6 Weeks Post Inoculation
Cultivar and Inoculation	No. of vessels assayed	Detection of P. gregata (%)a	Vessels Infected (%)b	Stem Severity (%)	No. of vessels assayed	Detection of P. gregata (%)	Vessels Infected (%)	Stem Severity (%)
Bell (R)c
Expt 1f
Ad	574	100	6.2	0	1074	100	6.9	13
Be	977	33	6.1	0	717	100	13.9	2
Control	769	0	1.2	0	786	0	0.0	0
Expt. 2f
A	870	100	1.1	0	816	100	3.0	10
B	956	33	1.6	0	698	100	29.9	2
Control	920	0	0.0	0	589	0	0.0	0
Corsoy 79 (S)
Expt. 1
A	704	0	8.8	0	714	100	49.2	45
B	727	100	3.2	0	734	100	17.8	12
Control	692	0	0.7	0	806	0	0.4	0
Expt. 2
A	730	100	1.3	0	736	100	65.4	73
B	779	33	3.2	0	748	100	14.1	7
Controlg	617	0	0.2	0	1023	0	0.0	0
IA 2008 (R)
Ah	750	33	1.3	0	1586	50	0.8	10
B	929	0	0.6	0	1252	100	5.7	23
Control	795	0	0.1	0	1196	0	0.0	0
LN 92-12033 (R)
A	730	0	0.0	0	1812	75	7.9	6
B	821	50	0.1	0	990	100	27.7	50
Control	906	0	0.0	0	1502	0	0.0	0
LN 92-12054 (S)
A	673	75	0.0	0	807	100	73.3	50
B	616	100	0.0	0	1224	100	25.2	60
Control	634	0	0.0	0	1698	0	0.0	0

^aDetection of P. gregata in plants. If P. gregata was not detected using standard PCR, a more sensitive real-time quantitative PCR assay was used.

^bThe average of the total number of vessels infected with P. gregata in a cross section.

^cReaction of soybean cultivars to P. gregata; R =  resistant, S =  susceptible.

^dPlants inoculated with Type A of P. gregata.

^ePlants inoculated with Type B of P. gregata.

^fTreatments were replicated three times (n = 3 plants/replication).

^gTreatments were replicated three times (n = 2 plants/replication).

^hTreatments were replicated three times (n = 4 plants/replication).