Table 2. Potential off target sites of gRNA-1.

	On/off target sites (5′-3′)	Locations	Annotations	Mismatches
On target	GGTGTTGTTGTCGTCGGCGTggg	exon	eyeless	-
Off target	GGTGTTGGTGTCGTCGGCGTcgg	exon	protocadherin fat 2 precursor	1 bp
	GTTGGTGTTGTCGTCGGCATcgg	exon	cytochrome c oxidase	3 bp
	ATTGGTGTTGTCGTCGGCATcgg	exon	cytochrome c oxidase	4 bp
	TGCGCCGTTGACGTCGGCGTtgg	intronic	-	5 bp

Genomic sequences of on/off target sites (uppercase) with PAM (NGG, lowercase), their locations, annotations, and the number of base pair differences are noted. Bold letters exhibit mismatched nucleotides. bp: base pairs.