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. 2014 May 30;9(5):e98544. doi: 10.1371/journal.pone.0098544

Figure 2. Overexpression of BRMS1 suppresses cell invasion but not cell proliferation in glioma cells.

Figure 2

A Twenty-four hours after transfection, the expression of BRMS1 in U251 and U87 glioma cells was evaluated by western blot. B, C CCK-8 cell proliferation assay was performed after BRMS1 overexpression in U251 and U87cells. D, E Matrigel cell invasion assay was performed after the overexpression of BRMS1 in U251 and U87 cells. The graph shows the percentage of cells invaded in 5 fields of view compared with the control group. F BRMS1 inhibits MMP-2 activity in U251 and U87 cells by zymography. G Western blot analysis of the relative protein levels of MMP-2, uPA and NF-κB in BRMS1 overexpression and control group of U251 and U87 cells. H Quantitative analysis of relative protein level of MMP-2, uPA, p27 and NF-κB in glioma U251 and U87 cells. All experiments were carried out in triplicate. Data are shown as mean ± SD. *P<0.05, **P<0.01.