Figure 1. Timeline for transplant experiments.

Donor mice undergo a GTT prior to islet isolation (∼3 days). Recipient mice are given an intraperitoneal injection of streptozotocin and diabetes occurrence is confirmed in approximately 5 days. Islets isolated from the donor mice are transplanted immediately into diabetic recipient mice. Recipient mice are checked for random-fed glucose levels at least 3 times a week until nephrectomy (28 days). GTT is performed on recipient mice on day 25 and nephrectomy on day 28 post-transplantation. Kidney with graft is collected for histology at nephrectomy.