Figure 6. AppΔapxIΔapxIIC cell culture supernatant and PRRSV effects on mRNA quantification of type I (IFNα, IFNβ) and type II (IFNγ) interferons.

qRT-PCR results expressed in relative expression (ΔΔCT) for IFNα (A), IFNβ (B) and IFNγ (C) in SJPL cells. The cells were mock infected or infected with 0.5 MOI of PRRSV for 4 hours then treated without or with AppΔapxICΔapxIIC cell culture supernatant for 48 hours. Poly (I:C) and LPS were used as positive controls. Data labeled with superscripts of different letters indicates that these sets of data are statistically different (P<0.05).