Figure 6.

E-cadherin is key downstream mediator of DDR2 S131C. H1703 cells were transfected with pEGFP-DDR2, pEGFP-DDR2-S131C, pEGFP-DDR2-T681I or empty vector, respectively. (A) QRT-PCR analysis was performed to determine E-cadherin, N-cadherin, MMP2 and MMP9 expression in H1703 cells. (B, C) E-cadherin and MMP2 protein expression in H1703 cells was determined by western blot analysis. The results are from 3 independent experiments. GAPDH protein expression was used as an internal control. * P < 0.05; ** P < 0.01.