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Ion spectra of lamivudine generated by Exactive-MS (A) with the inclusion of an in-source collision-induced dissociation scan event for fragment production and detection (B) .The 95 m/z fragment ion was likely formed through the loss of the oxathiol moiety and subsequent loss of a NH3 constituent. Retention time determination through the application of a mass filter with ± 5 ppm discrimination from theoretical mass of precursor and fragment ions (C).
