Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Cell Neurosci. 2014 Apr 23;60:63–71. doi: 10.1016/j.mcn.2014.04.002

Fig. 2.

Fig. 2

DYN expression in the dentate granule cell mossy fiber pathway was increased following chronic treatment with GluN2B-selective, but not non-subtype-selective NMDAR antagonists. Hippocampal slice cultures treated for the entire 17-21 day culture period with different classes of NMDAR antagonists were stained immunohistochemically with anti-DYN A antiserum using the ABC method and analyzed as described in the Materials and Methods. A) Representative hippocampal slice cultures as well as B) subjective analysis and C) quantitative densitometry revealed increased mossy fiber DYN immunoreactivity associated with the dentate granule cell mossy fiber pathway in organotypic hippocampal slice cultures treated with the GluN2B-selective NMDAR antagonists Ro25,6981 or ifenprodil, but not in cultures treated with the non-subtype-selective NMDAR antagonists APV or memantine. C inset) Hilar DYN immunoreactivity density (white box, 200 × 100 μm) was subtracted from background density in CA1 (black box, 200 × 100 μm). Abbreviations: g, dentate granule cell layer; h, hilus; m, molecular layer; sl, stratum lucidum; p, pyramidal cell layer. Arrowheads in A point to DYN-IR puncta. Bars indicate B, percentages or C, means ± SEM. The number of slice cultures is indicated in parentheses. Scale bar is for all panels in A, 500 μm. *, different than vehicle, p<0.05, ANOVA with Holm-Sidak post hoc comparison; **, different than vehicle, p<0.001, z-test.