Table 3.
Resistance genotypes a | PBP3 types b | n c | Sg d | Bla e |
PBP3 substitutions
f
|
||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
D |
S |
A |
M |
S |
P |
A |
I |
G |
A |
V |
R |
N |
A |
T |
V |
D |
A |
N |
|||||
350 | 357 | 368 | 377 | 385 | 392 | 437 | 449 | 490 | 502 | 511 | 517 | 526 | 530 | 532 | 547 | 551 | 554 | 569 | |||||
High-rPBP3 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Group III |
- |
1 |
|
|
N |
N |
|
|
T |
|
|
|
|
T |
|
|
Kg |
|
|
I |
|
|
S |
Group III-like |
- |
2 |
|
|
N |
N |
|
I |
T |
|
|
|
|
|
|
H |
|
|
S |
I |
|
|
|
Low-rPBP3 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
Group II |
A |
48 |
|
1 |
N |
|
|
I |
|
|
|
|
|
V |
|
|
Kh |
|
|
I |
|
|
S |
|
B |
19 |
|
5 |
|
|
|
|
|
|
|
V |
|
|
|
|
Kg |
|
|
I |
|
|
S |
|
C |
5 |
|
|
N |
|
|
I |
|
|
|
|
E |
|
|
|
Kh |
|
|
I |
|
|
S |
|
D |
17 |
|
|
N |
|
|
|
|
|
|
|
E |
|
|
|
Kg |
S |
|
|
|
|
|
|
F |
1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Kg |
|
|
|
|
|
|
|
H |
6 |
|
|
|
|
|
|
|
|
|
|
|
V |
|
|
Kh |
|
|
|
|
|
|
|
I |
4 |
|
|
N |
|
|
|
|
|
S |
|
|
V |
|
|
Kg |
|
|
I |
|
|
S |
|
J |
3 |
|
|
N |
|
|
|
|
|
|
|
|
T |
|
|
Kg |
|
|
I |
|
|
S |
|
K |
2 |
|
|
|
|
T |
|
|
|
|
|
|
T |
|
|
Kg |
|
|
|
|
|
|
|
L |
1 |
|
|
N |
|
|
|
|
|
|
|
E |
|
|
|
Kg |
|
|
I |
|
Di |
S |
|
M |
1 |
|
|
N |
|
|
|
|
|
|
|
|
V |
|
|
Kh |
|
|
I |
|
|
S |
|
N |
1 |
|
1 |
N |
|
|
|
|
|
S |
V |
|
|
|
|
Kg |
|
|
I |
|
|
S |
|
O |
1 |
|
|
|
|
|
|
|
|
|
|
|
T |
|
|
Kg |
|
|
I |
|
|
S |
|
P |
1 |
|
|
|
|
|
|
|
|
|
|
|
T |
|
|
Kg |
|
|
I |
|
|
|
|
Q |
1 |
|
|
|
|
|
|
|
|
|
|
E |
V |
|
|
Kh |
|
|
I |
|
|
S |
Group I |
- |
2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
H |
|
|
|
I |
|
T |
|
sPBP3 | z0 |
51 |
15 |
6 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
z4 |
9 |
1 |
|
N |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
I |
|
|
S |
|
z1 |
7 |
3 |
2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
I |
|
|
|
|
z6 |
3 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
I |
|
|
S |
|
z7 |
3 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
I |
|
T |
S |
|
z5 |
1 |
|
1 |
|
|
|
|
|
|
|
|
|
S |
|
|
|
|
|
|
|
|
|
|
z8 |
1 |
|
|
N |
|
|
|
|
|
|
|
|
T |
|
|
|
|
|
I |
|
|
S |
|
z9 |
1 |
|
|
N |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
I |
|
|
|
|
z10 |
1 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
I |
Ai |
|
|
|
z11 |
1 |
|
|
|
|
|
|
|
|
|
|
|
|
A |
|
|
|
|
I |
|
|
|
|
z12 |
1 |
|
|
|
|
|
|
|
Si |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
z13 | 1 | T |
aSee Table 1.
bPBP3 types according to Skaare et al.[11] (types A – G) and this study (types H – Q and z0 – z13). ‘-‘, not designated.
cn, No. of study isolates.
dSg, No. of isolates from the Susceptible group included in n.
eBla, No. of beta-lactamase positive isolates (all TEM-1) included in n.
fAmino acid substitutions in PBP3 (transpeptidase domain, 338–573) with the amino acid sequence of H. influenzae Rd KW20 [GenBank:U32793] as reference (z0). Essential substitutions in bold.
gN526K encoded by the DNA triplet AAG.
hN526K encoded by the DNA triplet AAA.
iNovel substitution.