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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2014 Apr 7;192(10):4560–4570. doi: 10.4049/jimmunol.1303461

Figure 3.

Figure 3

Epithelium-conditioned medium (ECM) induces MSC migration. (A) Air-liquid interface (ALI) culture system for airway epithelial cells. (B) Transwell assays for migration of MSCs. (C) Number of migrated cells in (B). (D) Levels of active TGF-β1 in ECM from un-challenged and challenged epithelial cells, n=3. (E) Number of migrated cells after ECM was treated with TGF-β1 neutralizing antibody (TGF-β1 Ab) or control antibody (IgG). (F) Number of migrated cells after ECM was treated with different doses of TβR1 inhibitor (SB505124). (G-H) Number of migrated cells after MSCs was directly treated with the addition of different doses of recombinant TGF-β1 into DEME in the lower chamber of Transwell (See B). Numbers of migrated cells were counted in five views (×20). Bars (C, E, and F) represent mean ± SEM of 3 independent experiments. *P<0.05 **P<0.01. BEC: bronchial epithelial cell.