Table 2.
Differential mRNA expression changes in healthy ASM cells after treatment with FCS or Dex + FCS
| mRNA |
FCS |
Dex + FCS |
||
|---|---|---|---|---|
| Microarray (FC) | qRT-PCR (FC) | Microarray (FC) | qRT-PCR (FC) | |
| FAM71F1 |
−1.7 (<.05) |
−1.62 (<.05) |
1.7 (<.05) |
1.56 (<.05) |
| METTL7A |
−1.9 (<.01) |
−0.85 (<.05) |
2.1 (<.01) |
5.77 (<.01) |
| PLSCR4 |
−1.5 (<.001) |
−0.33 (<.05) |
1.6 (<.001) |
6.47 (<.001) |
| TUBB3 |
1.5 (<.05) |
1.22 (<.05) |
−1.7 (<.001) |
−2.18 (<.05) |
| WDFY4 | −1.8 (<.05) | −3.60 (<.01) | 1.8 (<.05) | 2.01 (<.05) |
This table lists those mRNAs that were differentially expressed in healthy ASM cells following stimulation with FCS (2.5%) or treatment with dexamethasone (10−7 M) before stimulation with FCS (2.5%). Microarray data are denoted as the fold change against baseline control and is the mean of 9 individual patients. These values are derived from Additional file 1: Table S1, S2, S3, S4 and are for those genes that demonstrated an FDR of less than 0.05; associated P values are shown in parentheses. FC, Fold change.