PCR-SSP for RHD1227A (109 bp band).
Lanes 1–4, 6–9, RHD1227A allele detected in Del phenotype samples; Lane 5, non- RHD1227A allele gene detected in Del phenotype samples; lane 10, Rh-negative control sample; lane 11, sample with a normal RHD gene; lane 12, H2O control. A representative example of 155 Del phenotyped cases is shown.