Table 1.
Coding DNA Sequence (CDS) identifications of let- genes
| Gene | Allele | Allele mutation | Molecular identity | Support | Confirmation status | Human ortholog | Associated human conditions | References |
|---|---|---|---|---|---|---|---|---|
| lin-6/mcm-4 | h92 | C > * | Mini chromosome maintenance | RNAi | Confirmed1 | MCM4 | Natural killer cell and glucocorticoid deficiency with DNA repair defect | [39] |
| let-354/dhc-1 | h79 | Q > * | Dynein heavy chain | Both | Confirmed1 | DYNC1H1 | Charcot-Marie-Tooth disease, Mental retardation, Spinal muscular atrophy | [40–42] |
| let-502/rock | h392 | Q > * | Rho associated kinase | RNAi | Confirmed1 | ROCK1 | [43] | |
| let-363/tor | h98 | Splice variant | Tor kinase | Both | Confirmed1 | MTOR | pancreatic neuroendocrine tumors | [44, 45] |
| h420a | Q > * | Confirmed3 | ||||||
| h502a | Splice variant | Confirmed3 | ||||||
| let-603/air-2 | h289 | W > * | Aurora-related serine/threonine kinase | Both | Confirmed1 | AURKA | Susceptibility to colon cancer | [46] |
| let-512/vps-34 | h797 | P > S | phosphoinositide 3-kinase | Confirmed1 | PIK3C3 | [47] | ||
| let-381/foxf | h107 | splice variant | Forkhead transcription factor F | K.O. | Confirmed1 | FOXF2 | [48] | |
| let-607/bZip | h402 | Q > * | Leucine zipper transcription factor | Both | Confirmed1 | CREB3L3 | [49] | |
| let-504/E01A2.4 | h448 | M > I | NFkB activating protein | Both | Confirmed1 | NKAP | ||
| let-152/ccb-1 | h685 | W > * | Calcium channel subunit | Confirmed2 | CACNB2 | Brugada syndrome 4 | ||
| let-355/hel/T05E8.3 | h81 | Y > * | DEAD/H helicase | RNAi | Confirmed2 | DHX33 | ||
| let-362/rhel/Y71G12B.8 | h86 | R > * | DEAD/H RNA helicase | RNAi | Confirmed2 | DDX27 | ||
| let-366/aars-2 | h112 | Q > * | Alanine tRNA synthetase | RNAi | Confirmed2 | AARS | Charcot-Marie-Tooth disease | [50] |
| let-368/inx-12 | h121 | W > * | Innexin gap junction | K.O. | Confirmed2 | |||
| let-370/coq-1 | h128 | G > E | hexaprenyl pyrophosphate synthetase | K.O. | Confirmed2 | PDSS1 | Coenzyme Q10 deficiency, Parkinson’s disease | [51] |
| let-389/nars-1 | h680 | G > E | Asparagine tRNA synthetase | Both | Confirmed2 | NARS | ||
| let-396/fars-1 | h217 | Q > * | Phenylalanine tRNA synthetase | RNAi | Confirmed2 | FARSA | ||
| let-522/hlh-2 | h735 | W > * | Helix loop helix transcription factor | Both | Confirmed2 | TCF3 | Acute lymphoblastic leukemia | |
| let-529/asd-2 | h238 | Q > * | KH domain containing RNA binding protein | RNAi | Confirmed2 | QKI | Mental retardation | |
| let-575/ptr-2 | h345 | W > * | Sterol sensing domain protein | RNAi | Confirmed2 | PTCHD1 | Autism spectrum disorders | [52–54] |
| let-585/inx-13 | h784 | W > * | Innexin gap junction | RNAi | Confirmed2 | |||
| let-595/imb-1 | h353 | R > * | Importin | RNAi | Confirmed2 | KPNB1 | ||
| let-598/F27C1.6 | h213 | Q > * | U3 small nucleolar ribonucleoprotein | RNAi | Confirmed2 | UTP14C | ||
| let-599/nath-10 | h290 | L > F | N-acetyl transferase | Both | Confirmed2 | NAT10 | ||
| let-608/ncbp-1 | h706 | Q > * | Nuclear cap binding protein | RNAi | Confirmed2 | NCBP1 | ||
| let-611/C48E7.2 | h756 | Q > * | RNA polymerase III subunit | RNAi | Confirmed2 | POLR3C | ||
| let-612/apm-1 | h466 | splice variant | Adaptin subunit | RNAi | Confirmed2 | AP1M1 | ||
| let-365/sep-1 | h108 | W > * | Separase | Both | Confirmed2 | ESPL1 | Breast cancer oncogene | |
| let-364/mat-1 | h104 | S > F | Anaphase promoting complex subunit | RNAi | Confirmed2 | CDC27 | ||
| let-101/npp-6 | h242 | W > * | Nuclear pore complex protein | Both | Confirmed2 | NUP160 | ||
| let-106/hcp-6 | h787 | C > Y | Condensin subunit | Both | Confirmed2 | NCAPD3 | ||
| let-379/tag-345 | h127 | W > * | Nucleolar protein complex member | RNAi | Confirmed2 | WDR12 | ||
| let-503/R12E2.2 | h313 | Q > * | Protein of unknown function | K.O. | Confirmed2 | SUCO | ||
| let-517/spg-7 | h264 | G > E | Metalloprotease | Both | Confirmed2 | AFG3L2 | Spastic ataxia, Spinocerebellar ataxia | |
| let-597/hcp-4 | h349 | E > * | Holocentromeric protein | RNAi | Confirmed2 | CENPC | ||
| let-630/Y110A7A.19 | h355b | R > * | Pentatricopeptide repeat containing protein | RNAi | Confirmed2 | PTCD3 | ||
| h782b | W > * | Confirmed2 | ||||||
| let-646/pat-10 | h233 | G > E | Troponin C | RNAi | Confirmed2 | TNNC1 | Cardiomyopathy | |
| let-526 | h799c | Q > * | SWI/SNF complex subunit | Both | Confirmed3 | ARID1A | Mental retardation | |
| h405c | W > * | Confirmed3 | ||||||
| let-129/zfh-2 | h379 | Q > * | zinc finger homeobox protein | Both | Prediction | ZFHX3, ZFHX4 | Susceptibility to prostate cancer, Ptosis | |
| let-147/rnp-6 | h463 | G > E | RNA splicing factor | RNAi range | Prediction | PUF60 | Verheij syndrome | |
| let-373/unc-73 | h234 | Del | Guanine nucleotide exchange factor | Both | Prediction | TRIO | ||
| let-377/lim-7 | h110 | W > * | LIM homeodomain protein | K.O. | Prediction | ISL2 | ||
| let-378/dnj-21 | h124 | G > E | DnaJ domain containing protein | RNAi | Prediction | DNAJC15 | ||
| let-380/knl-2 | h80 | W > * | Centromeric protein | Both | Prediction | |||
| let-382/nuo-2 | h82 | Q > * | Mitochondria complex I subunit | Both | Prediction | NDUFS3 | Leigh syndrome, Mitochondrial complex I deficiency | |
| let-383/T21G5.6 | h115 | W > * | Protein of unknown function | Prediction | ||||
| let-384/C06A5.1 | h84 | Q > * | Integrator subunit | RNAi | Prediction | INTS1 | ||
| let-385/teg-4 | h85 | splice variant | splicing factor | RNAi | Prediction | SF3B3 | ||
| let-386/dbr-1 | h117 | G > E | RNA lariat-debranching enzyme | RNAi range | Prediction | DBR1 | ||
| let-391/tag-146 | h91 | Q > * | Uncharacterized zinc finger protein | K.O. | Prediction | |||
| let-397/rpb-5 | h228 | Q > * | RNA polymerase II subunit | RNAi | Prediction | POLR2E | ||
| let-400/prpf-4 | h269 | D > G | Pre-mRNA processing factor | RNAi | Prediction | PRPF4B | [55] | |
| let-509/unc-73 | h142 | W > * | Guanine nucleotide exchange factor | Both | Prediction | TRIO | ||
| let-527/nhr-23 | h207 | R > Q | Nuclear hormone receptor | Both | Prediction | RORC | ||
| let-534/ahcy-1 | h260 | Q > * | S-adenosylhomocysteine hydrolase | Both | Prediction | AHCY | Hypermethioninemia | |
| let-581/unc-11 | h725 | A > V | clathrin adaptor protein | RNAi; Range | Prediction | PICALM | Acute lymphoblastic leukemia, Acute T-cell lymphoblastic leukemia | |
| let-601/cuti-1 | h281 | Q > * | Cuticle regulatory protein | Both | Prediction | |||
| let-602/T09B4.9 | h283 | W > * | translocase | RNAi | Prediction | TIMM44 | ||
| let-604/mdt-18 | h293 | splice variant | Mediator subunit | RNAi | Prediction | MED18 | ||
| let-605/cye-1 | h312 | W > * | E-type cyclin | Both | Prediction | CCNE1 | ||
| let-614 | h138 | Tested against F27C1.3 but did not confirm | ||||||
| let-376 | h130 | Tested against F55F8.3 but did not confirm | ||||||
| let-375 | h241 | Tested against imb-1 but did not confirm | ||||||
| let-387 | h87 | Tested against pnk-1 but did not confirm | ||||||
| let-515 | h730 | Tested against rpl-13 but did not confirm | ||||||
| let-501 | h714 | Tested against rpl-4 but did not confirm | ||||||
| let-361 | h97 | no candidate | ||||||
| let-531 | h733 | no candidate | ||||||
| let-576 | h816 | no candidate | ||||||
| let-518 | h316 | no candidate | ||||||
| let-523 | h751 | no candidate | ||||||
| let-525 | h874 | no candidate | ||||||
| let-584 | h743 | no candidate |
The asterisk (*) signify a stop codon. Support column describes whether the CDS are lethal when treated with RNAi or a knock-out (K.O.) allele, or both. RNAi Range signifies RNAi lethal phenotype show varying degree of penetrance. Confirmation status notes: 1Confirmed by previous publication. 2Confirmed by sequencing 2nd allele. 3Confirmed by complementation testing. Annotation of human orthologs and associated human conditions are from the literature and public databases such as WormBase and OMIM. The genes are sorted first by confirmation status and then by genomic coordinates.
a let-103 (h420) and let-631 (h502) have collapsed into let-363.
b let-596 (h782) and let-630 (h355) both confirmed by sequencing a second allele and failed to complement each other. Thus, these two are collapsed into let-630.
c let-104 (h799) and let-519 (h405) have collapsed into let-526.