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. 2014 May 21;4(5):e004357. doi: 10.1136/bmjopen-2013-004357

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/3.0/

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BOLD signal intensity changes during task-related fMRI in relation to the specific condition, that is, infusion or to a contrast, that is, low versus full bladder volume, during two (healthy controls) or three (patients with non-neurogenic LUTS) visits. Investigation of these changes will focus on supraspinal regions of interest (ROI) that are known from the existing literature, for example, pons, insula, anterior cingulate cortex, thalamus, hypothalamus, supplementary motor area and prefrontal cortex. However, the precise selection of ROIs will be based on the coordinates of the peak activations during task-related fMRI taken from the Montreal Neurological Institute (MNI) space.