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Sickling of red blood cells (RBCs) from homozygous (HbSS) patients with sickle cell disease (SCD). RBCs were incubated for 20 min in tonometers at 2% haematocrit at the oxygen tension indicated. Aliquots were then fixed in saline pre-equilibrated at the same oxygen tension with the addition of glutaraldehyde (0.3%). Morphological sickling was determined by light microscopy. Histograms represent means ± S.E.M., n = 3.
