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. 2014 Jun;53(1-2):21–26. doi: 10.1016/j.bcmd.2014.02.004

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Fig. 2 — Effect of o-vanillin on the potassium permeability of RBCs from patients with SCD. Activity of the K⁺–Cl⁻ cotransport (KCC) was determined as the Cl⁻-dependent K⁺ influx in fully oxygenated RBCs from homozygous HbSS individuals. Activity of P_sickle was determined as the deoxygenation-induced Cl⁻-independent K⁺ influx in N₂; that of the Gardos channel was determined as the clotrimazole (CLT; 5 μM)-sensitive K⁺ influx—both in fully deoxygenated RBCs. o-Vanillin (5 mM) was present either during the period of measurement of transporter activity only, or, in addition, during 30 min pre-incubation as well. Flux measurements are normalised to those measured in control RBCs in the absence of o-vanillin and presented as a percentage of their maximal value, which, for HbSS were 2.35 ± 0.45 mmol (l cells h)⁻¹ for KCC in air, 1.02 ± 0.22 for P_sickle and 5.80 ± 1.16 for the Gardos channel. Data represent means ± S.E.M. for 4–7 determinations.